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Water: Bioindicators

Cryptosporidium (and Giardia) in Water by Filtration/IMS/FA

Method 1622

EPA Method 1622 is a performance-based method applicable to the determination of Cryptosporidium in aqueous matrices. EPA Method 1622 requires filtration, immunomagnetic separation of the oocysts from the material captured, and an immunofluorescence assay for determination of oocyst concentrations, with confirmation through vital dye staining and differential interference contrast microscopy.


To support future regulation of protozoa in drinking water, the Safe Drinking Water Act Amendments of 1996 require the U.S. Environmental Protection Agency (EPA) to evaluate the risk to public health posed by drinking water contaminants, including waterborne parasites, such as Cryptosporidium. To implement these requirements, EPA must assess Cryptosporidium occurrence in raw surface waters used as source waters for drinking water treatment plants. EPA Method 1622 was developed to support this assessment.

Method 1622 (PDF) (75 pp, 1.3MB, About PDF) (EPA-821-R-01-026) (April 2001)

For Further Information

If you need additional information or have questions pertaining to this draft method, you may contact the Engineering and Analysis Division at 202-566-1000.

Method 1623

Method 1623: Cryptosporidium and Giardia in Water by Filtration/IMS/FA (PDF) (76 pp, 1.2MB; About PDF)
A performance-based method applicable to the determination of Cryptosporidium and Giardia in aqueous matrices. (April 2001)

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