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Water: Long Term 2 Enhanced Surface Water Treatment Rule

Laboratory Certification for Analysis of Cryptosporidium under the Safe Drinking Water Act

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The purpose of laboratory oversight is to identify laboratories that can reliably measure the occurrence of Cryptosporidium in surface water using approved methods.

For the first round (2006-2012) of Cryptosporidium monitoring under the Long Term 2 Enhanced Surface Water Treatment Rule (LT2ESWTR), EPA’s Office of Ground Water and Drinking Water (OGWDW) assumed responsibility for the approval and ongoing oversight of the laboratories that supported Public Water Systems (PWSs). In preparation for the second round of LT2ESWTR monitoring scheduled to begin in April 2015, OGWDW’s Technical Support Center (TSC) is working with EPA Regions and States to integrate Cryptosporidium laboratory approval/oversight into existing State certification/accreditation programs. State oversight of Cryptosporidium laboratories is provided for in the LT2ESWTR and is consistent with the laboratory oversight approach for all other regulated analytes. EPA Cryptosporidium laboratory approval granted by TSC does not carry over to the second round of LT2ESWTR Cryptosporidium monitoring that begins in 2015.

Laboratory Certification Process

Laboratories wishing to support the LT2ESWTR Cryptosporidium monitoring program during the second round will need to:

States interested in integrating Cryptosporidium laboratory approval into their certification/accreditation program should:

Carrie Miller, Cryptosporidium Laboratory Technical Liaison
U.S. Environmental Protection Agency
Office of Ground Water and Drinking Water, Technical Support Center
26 West Martin Luther King Dr. (MS-140)
Cincinnati, Ohio 45268
Fax number: (513) 569-7191
E-mail: Carrie Miller (miller.carrie@epa.gov)

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Certified Laboratories

Laboratories supporting the LT2ESWTR Cryptosporidium monitoring program during the second round

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Federal Register Notices

Recommended Resources

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Training Modules

The training modules provide guidance and instruction to utility and laboratory personnel on LT2ESWTR sample collection and analysis.
  1. Adobe Flash Player is required to view the modules.
  2. Before viewing the training modules, please review the Module Access Directions.
  • Sample Collection (56.7 MB ZIP File)
    This module details the sampling procedures for bulk sample collection and field filtration, and provides instruction on proper sample packing and shipping.
  • Basic Microscopy (18.7 MB ZIP File)
    This module assists analysts in the proper use and maintenance of a microscope as used to detect Cryptosporidium and Giardia in Method 1623.
  • Microscopic Identification (13.4 MB ZIP File)
    This module helps analysts properly identify and characterize Cryptosporidium and Giardia using Method 1623. The module includes characterization by FITC, DAPI, and DIC of positive control organisms and interfering organisms.
  • Immunomagnetic Separation (25.6 MB ZIP File)
    This module details the immunomagnetic separation (IMS) procedure used in EPA Method 1623 to separate Cryptosporidium oocysts and Giardia cysts from extraneous debris in a water sample.

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