Biological Pollutants in Ambient Water
- Method 1609: Enterococci in Water by Quantitative Polymerase Chain Reaction (qPCR) with Internal Amplification Control (IAC) Assay
- Analytical Methods for Biological Pollutants in Wastewater and Sewage Sludge
- Other Biosolids Methods
- Rapid Microbiological Methods for Ambient Waters
You will need Adobe Reader to view some of the files on this page. See EPA's PDF page to learn more.In September 2008, we completed two reports:
- False positive and negative rates for fecal coliforms (EPA Methods 1680 and 1681) in biosolids (PDF) (30 pp, 158K)
- Validation of enterococci (EPA Methods 1106.1 and 1600) and E. coli (EPA Methods 1103.1 and 1603) in CSO matrices (PDF) (66 pp, 347K)
Guidelines Establishing Test Procedures for the Analysis of Pollutants
Analytical Methods for Biological Pollutants in Wastewater and Sewage Sludge
September 29, 2007
We issued new methods that use culture-based approaches to detect enterococci and Escherichia coli (E. coli) in wastewater. Additional tests will also identify Salmonella and fecal coliform bacteria in sewage sludge.
|1103.1||Escherichia coli (E. coli) in Water by Membrane Filtration Using membrane-Thermotolerant Escherichia coli Agar (mTEC) (PDF) (45 pp, 824K)|
|1106.1||Enterococci in Water by Membrane Filtration Using membrane-Enterococcus-Esculin Iron Agar (mE-EIA) (PDF) (41 pp, 755K)|
|1600||Membrane Filter Test Method for Enterococci in Water (PDF) (42 pp, 573K)|
|1603||Escherichia coli (E. coli) in Water by Membrane Filtration Using Modified membrane-Thermotolerant Escherichia coli Agar (Modified mTEC) (PDF) (42 pp, 504K)|
|1680||Fecal Coliforms in Sewage Sludge (Biosolids) by Multiple-Tube Fermentation using Lauryl Tryptose Broth (LTB) and EC Medium (PDF) (50 pp, 172K)|
|1681||Fecal Coliforms in Sewage Sludge (Biosolids) by Multiple-Tube Fermentation using A-1 medium (PDF) (51 pp, 242K)|
|1682||Salmonella in Sewage Sludge (Biosolids) by Modified Semisolid Rappaport-Vassiliadis (MSRV) Medium (PDF) (48 pp, 284K)|
Other Biosolids Methods
Note: Many of these methods are drafts.
Rapid Microbiological Methods for Ambient Waters
We are making available a method that uses a genetic quantitative polymerase chain reaction (qPCR) approach to detect enterococci in ambient waters. The method will be updated following multi-laboratory validation in marine and fresh ambient waters. The presence of a method here does not imply a regulatory requirement for its use.
|1609||Enterococci in Water by Quantitative Polymerase Chain Reaction (qPCR) with Internal Amplification Control (IAC) Assay (PDF) (72 pp, 988K) (EPA-820-R-13-005; March 2013)
Calculation Spreadsheet (Excel, 128K)
|1611||Enterococci in Water by TaqMan® Quantitative Polymerase Chain Reaction (qPCR) Assay (PDF) (56 pp, 413K) (EPA-821-R-12-008, October 2012)|
Calculation Spreadsheet (Excel, 89K)
|Method B Bacteroidales in Water by TaqMan® Quantitative Polymerase Chain Reaction (qPCR) Assay (PDF) (111 pp, 8.6MB)|
- Acceptability of the EPA qPCR Test at Your Beach (PDF) (4 pp, 36K) (EPA 820-R-13-012; December 2013)
- Detection and Quantification Limits of EPA Enterococcus qPCR Methods (PDF) (7 pp, 114K) (EPA 820-R-13-013; December 2013)
Videos About qPCR
- qPCR training videos developed by Michigan State University. Enterococci Testing Video Series: collecting samples, rules of sampling, lab supplies, lab safety, blank membrane filtration, water sample filtration, DNA extraction, preparing assay mix, and preparing tray
- Southern California Coastal Water Research Project. Several videos, including qPCR training and microbial source tracking methods.
- “qPCR in your lab” video from Water Environment Research Foundation. Introduces a rapid method for monitoring pathogen indicators.